Hard Practice — Structure, Test & Base-pairing Reasoning

Reason from evidence to structure: reducing-sugar logic, glucose reactions (what each proves), base-pairing and melting-temperature arguments, and protein structure/enzyme logic.

  • Free anomeric CHO-CHO/C=O-C{=}O reduces Tollens'/Fehling'; sucrose is non-reducing.
  • A-T (2 H-bonds), G-C (3 H-bonds) \Rightarrow higher G-C content raises the DNA melting temperature.
  • Denaturation loses 2^\circ/3^\circ structure, not the peptide (1^\circ) sequence.